Composite
Part:BBa_K1321331:Design
Designed by: Michael Florea Group: iGEM14_Imperial (2014-10-08)
araC-Pbad in pSEVA331-Bb
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4089
Illegal suffix found in sequence at 1218 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4089
Illegal SpeI site found at 1219
Illegal PstI site found at 1233
Illegal NotI site found at 1226
Illegal NotI site found at 4095 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4089
Illegal BamHI site found at 1144 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4089
Illegal suffix found in sequence at 1219 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4089
Illegal XbaI site found at 4104
Illegal SpeI site found at 1219
Illegal PstI site found at 1233
Illegal NgoMIV site found at 2408
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
BBa_K1321331 was created by restricting BBa_K808000 and BBa_K1321300 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligation reaction into E.coli DH10B.