Composite

Part:BBa_K1321331:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)

araC-Pbad in pSEVA331-Bb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4089
    Illegal suffix found in sequence at 1218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4089
    Illegal SpeI site found at 1219
    Illegal PstI site found at 1233
    Illegal NotI site found at 1226
    Illegal NotI site found at 4095
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4089
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4089
    Illegal suffix found in sequence at 1219
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4089
    Illegal XbaI site found at 4104
    Illegal SpeI site found at 1219
    Illegal PstI site found at 1233
    Illegal NgoMIV site found at 2408
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

BBa_K1321331 was created by restricting BBa_K808000 and BBa_K1321300 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligation reaction into E.coli DH10B.


Source

References